THE PREPARATION OF FROZEN-DRIED TISSUE FOR ELECTRON MICROSCOPY
نویسندگان
چکیده
منابع مشابه
The Preparation of Frozen-dried Tissue for Electron Microscopy
A method is described for the preparation of liver by freezing and drying which avoids the formation of ice crystals visible with the electron microscope. Such dried preparations after postfixation may be stained with numerous reagents. They may also be stained for cytochemical study of ribonucleic and desoxyribonucleic acids, and for glycogen. Details of the methods are given, together with a ...
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To explore the problem of artefacts which may be produced during usual fixation, dehydration, and embedding, the authors have examined pancreas, liver, and bone marrow frozen at about -180 degrees C., dried, at -55 to -60 degrees C., embedded in methacrylate, sectioned, and floated on a formol-alcohol mixture. By these treatments the labile structure of living cells can be fixed promptly and em...
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In general the SEM sample preparation techniques are more or less similar to the metallographic sample preparation technique for optical microscopy, because both the microscopes reveals the surface topography of sample. In SEM a focussed beam of electrons scans over the specimen surface and the electrons emitted from the sample surface controls the brightness of the CRT spot which also scans th...
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An aspect of bacteriophage morphology which never fails to excite speculation is the function of that tail which is characteristic of most of the T-bacteriophages and, so far as is known, of all other bacteriophages. Until recently nothing has been known about the tail, and there has been no reason to call one end a tail and the other a head, except for the obvious similarity in appearance to h...
متن کاملElectron microscopy of frozen hydrated biological specimens.
The use of frozen hydrated specimens for molecular structure determination is limited primarily by radiation damage. The radiation damage effect in frozen hydrated catalase crystals has been measured in terms of the loss of electron diffraction. The results show an improvement for this type or:specimen relative to wet hydrated or to glucose embedded catalase crystals at room temperature. Bright...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1956
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.2.4.37